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KMID : 0829220100340020073
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Korean Journal of Oral and Maxillofacial Pathology
2010 Volume.34 No. 2 p.73 ~ p.81
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Identification of Putatively Virulent Candida Species in Saliva Obtained from Elderly Individuals in Gwangju
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Ko Young-Jong
Lim Won-Bong
Kim Ji-Sun
Kim In-Ae
Kwon Hyuk-Il
Kim Ok-Soo
Kim Byung-Gook
Kim Seon-Mi
Youn Suk-Ja
Kang Byung-Chul
Lim Hoi-Soon
Kim Mi-Sook
Kim Ok-Joon
Choi Hong-Ran
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Abstract
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Candida albicans and their associated Candida species are opportunistic pathogens which exists as normal flora in the oral cavities of healthy individuals. In response to physiological changes in the host, these yeasts can become pathogenic, resulting in oral candidiasis. The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidiasis. The presently available culture and biochemical methods for detection and species identification of Candida are time-consuming and lack the required sensitivity and specificity. In this study, we have established a seminested PCR (snPCR) using universal and species-specific primers for detection of Candida species in saliva. The universal outer primers amplified the 3end of 5.8S ribosomal DNA (rDNA) and the 5end of 28S rDNA, including the internally transcribed spacer 2 (ITS2), generating
350- to 410-bp fragments from the four commonly encountered Candida spp., viz., C. albicans, C. tropicalis, C. glabrata, and C. parapsilosis. The saliva from 331 healthy and, over 50 years of aged people lived in Dong-gu, Gwangu city, was collected. Total DNA were extracted by Hoffman-Winston yeast total DNA prep. method and performed the snPCR. Results appeared to be negative on 292 people (88.2%), however, 26 people (7.9%) were positive Candida albicans, 6 people (1.8%) were positive Candida glabrata, 5 people (1.5%) were positive Candida tropicalis, and only 2 person (0.6%) were positive Candida parapsilosis. These result showed that detection and identification of Candida species could be established by saliva analysis, so that snPCR on saliva is useful method of diagnosis of clinical fields.
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KEYWORD
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C. albiacans, Candida, Candidiasis, C. glabrata, C. parapsi, C. tropicalis, saliva, sn-PCR
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